Almejas de importancia comercial del Food quality research center

Registros

Los datos en este recurso de registros biológicos han sido publicados como Archivo Darwin Core(DwC-A), el cual es un formato estándar para compartir datos de biodiversidad como un conjunto de una o más tablas de datos. La tabla de datos del core contiene 655 registros.

Este IPT archiva los datos y, por lo tanto, sirve como repositorio de datos. Los datos y los metadatos del recurso están disponibles para su descarga en la sección descargas. La tabla versiones enumera otras versiones del recurso que se han puesto a disposición del público y permite seguir los cambios realizados en el recurso a lo largo del tiempo.

Versiones

La siguiente tabla muestra sólo las versiones publicadas del recurso que son de acceso público.

¿Cómo referenciar?

Los usuarios deben citar este trabajo de la siguiente manera:

Cayupi Angulo A, Araneda Tolosa C, Larraín Barth M A (2024). Almejas de importancia comercial del Food quality research center. Version 1.1. Universidad de Chile. Occurrence dataset. http://gbif-chile.mma.gob.cl/ipt/resource?r=fqrc_clams&v=1.1

Derechos

Los usuarios deben respetar los siguientes derechos de uso:

El publicador y propietario de los derechos de este trabajo es Universidad de Chile. Esta obra está bajo una licencia Creative Commons de Atribución/Reconocimiento-NoComercial (CC-BY-NC 4.0).

Registro GBIF

Este recurso ha sido registrado en GBIF con el siguiente UUID: 4673875f-24c0-49a0-9807-0e823c2089c0.  Universidad de Chile publica este recurso y está registrado en GBIF como un publicador de datos avalado por GBIF Chile.

Palabras clave

Occurrence; Clams; Chile; Veneridae; Semelidae; Mactridae; Psammobiidae; Specimen

Contactos

Cobertura geográfica

This clams were collected on the coasts of Chile.

Cobertura taxonómica

N/A

Cobertura temporal

Datos del proyecto

The FONDEF ID18I10025 project has as goal to develop a multi-locus diagnostic method based on DNA meta-barcoding technology for the identification of clam species found in Chile, as the first stage to achieve forensic standards and support the traceability and competitiveness of this resource destined to be exported. The development of this capacity is important for Chile because clams are the second in volume of landings within the mollusks obtained from fisheries. Nine species of clams of commercial importance have been described in the country Ameginomya antiqua (Reticulated clam), Leukoma thaca (Taca clam), Tawera elliptica (Baby clam), Mulinia edulis (Taquilla), Semele solida (Tumbao), Gari solida (Culengue ), and three white clams Eurhomalea rufa, E. lenticularis and E. exalbida. These products have been exported to the European Union (EU), whose regulation 1379/2013 requires to declare, among other information, the commercial and the scientific name of the species to be declared. When clams are processed, the shells are removed making it impossible to know their species using morphological characters. The lack of reliable methods for the identification of the clam species from Chile has negatively affected its export to the EU. At the end of the project, the production result was achieved (“Diagnostic method for the identification of clam species from Chile”), along with other committed results (publication in a specialized magazine of the aquaculture sector and presentation of advances in national and international meetings). The 4 milestones committed were 100% met. During Milestone 1, samples of the nine clam species were collected from Iquique to Punta Arenas (~ 3,700 km) and were identified by an expert taxonomist and extracted to obtain the DNA. During Milestone 2, the "barcode" sequence reference database was constructed. Testing 29 pairs of universal primers for previously published “barcode” genes, the 16S rDNA, Histone 3, Histone 4, ITS-1 and ITS-2 genes were selected because they showed the best performance. These were sequenced by Sanger in 20 specimens of each of the nine target species, and their edited and curated sequence were indexed to be used as a local database with the BLAST tool ("Basic Local Alignment Search Tool"). In Milestone 3, using bioinformatic methods and the “barcode” sequences, five “mini-barcode” primer pairs were developed that allowed to obtain sequences with size between 126 to 220 bp to analyze process products. During Milestone 4, the “mini-barcodes” were standardized to be used in the iSeq 100 Sequencing System (Illumina). The performance of the method was evaluated through the diagnostic statistics of Sensitivity (S) indicating the ability of the method to correctly identify the species and Specificity (E) that shows the ability of the method to correctly exclude clams of other species. The method showed optimal performance identifying the nine species of clams analyzed (S = E = 1.0). Standardized Operating Procedures (SOPs) for analysis were developed to apply the method to fresh clam samples. This result is the first stage in the development of a reliable method for the identification of the nine clams of commercial interest in Chile with forensic standards. It is expected in a continuity project to take the method from the current TLR4 (laboratory tested technology) to a TLR7 by testing the technology in a real environment.

Métodos de muestreo

shopping at markets and natural collection by scuba diving

Descripción de la metodología paso a paso:

1. Specimen sampling Taxonomic identification Tissue sampling Sex identification DNA extraction Standard PCR Amplicon purification Sanger sequencing Assembly & alignment sequencher Barcoding gap & mini-barcoding design Terting mini-barcodes in local & NCBI DB Developing of local indexed DB

Datos de la colección

Referencias bibliográficas

1. Vargas Manríquez C , Vásquez García S , Larraín Barth M A y Araneda Tolosa C (2022). Desarrollo de una herramienta molecular para la identificación de especie en almejas de Chile: Implicancias en autenticidad, inocuidad y conservación.

Metadatos adicionales