Description
This resource represents to date the largest collection of basidiomycetes yeasts isolated from lichen thalli and the first collection of yeasts associated with Peltigera lichens. The Microbial Ecology Lab (Universidad de Chile) collection comprises 180 basidiomycetes yeasts isolated and identified from Peltigera lichen thalli (P. frigida, P. fuscopraetextata, P. rufescens and Peltigera ponojensis/monticola 11) collected from southern Chile. The isolates were identified by sequencing the ITS marker and subsequent phylogenetic analyses. A great diversity of basidiomycetes genera was found, belonging to the classes Agaricostilbomycetes, Cystobasidiomycetes, Microbotryomycetes and Tremellomycetes and to the orders Agaricostilbales, Cystobasidiales, Cystofilobasidiales, Filobasidiales, Holtermanniales, Sporidiobolales and Tremellales. Georeferencing included for all specimens.
Data Records
The data in this occurrence resource has been published as a Darwin Core Archive (DwC-A), which is a standardized format for sharing biodiversity data as a set of one or more data tables. The core data table contains 180 records.
1 extension data tables also exist. An extension record supplies extra information about a core record. The number of records in each extension data table is illustrated below.
This IPT archives the data and thus serves as the data repository. The data and resource metadata are available for download in the downloads section. The versions table lists other versions of the resource that have been made publicly available and allows tracking changes made to the resource over time.
Versions
The table below shows only published versions of the resource that are publicly accessible.
How to cite
Researchers should cite this work as follows:
Perez Y, Serey N, Millanes A M, Lizana N, Almendras K, Orlando J (2023). BASIDIOMYCETE YEASTS ISOLATED FROM PELTIGERA LICHENS OF SOUTHERN CHILE. Version 1.5. Instituto de Biodiversidad de Ecosistemas Antárticos y Subantárticos (BASE). Occurrence dataset. https://gbif-chile.mma.gob.cl/ipt/resource?r=yeast_pelt_basi&v=1.5
Rights
Researchers should respect the following rights statement:
The publisher and rights holder of this work is Instituto de Biodiversidad de Ecosistemas Antárticos y Subantárticos (BASE). This work is licensed under a Creative Commons Attribution (CC-BY 4.0) License.
GBIF Registration
This resource has been registered with GBIF, and assigned the following GBIF UUID: 6322f1dd-67c5-4938-bc43-ca66ea3cf102. Instituto de Biodiversidad de Ecosistemas Antárticos y Subantárticos (BASE) publishes this resource, and is itself registered in GBIF as a data publisher endorsed by GBIF Chile.
Keywords
culture; Chile; lichen; phylogeny; yeasts; basidiomycetes; Peltigera
Contacts
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Geographic Coverage
Region of Aysen del General Carlos Ibañez del Campo, Chile Region of Magallanes y de la Antártica Chilena, Chile
| Bounding Coordinates | South West [-54.955, -73.652], North East [-45.529, -67.633] |
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Taxonomic Coverage
No Description available
| Kingdom | Fungi |
|---|---|
| Phylum | Basidiomycota |
| Class | Agaricostilbomycetes, Cystobasidiomycetes, Microbotryomycetes, Tremellomycetes |
| Order | Agaricostilbales, Cystobasidiales, Cystofilobasidiales, Filobasidiales, Holtermanniales, Sporidiobolales, Tremellales |
| Family | Bulleraceae, Bulleribasidiaceae, Chionosphaeraceae, Cystobasidiaceae, Filobasidiaceae, Holtermanniaceae, Mrakiaceae, Piskurozymaceae, Sporidiobolaceae |
| Genus | Cystobasidium, Filobasidium, Fonsecazyma, Genolevuria, Goffeauzyma, Holtermanniella, Naganishia, Pseudotremella, Rhodotorula, Solicoccozyma, Tausonia |
| Species | Cystobasidium cf. laryngis, Cystobasidium cf. pinicola, Cystobasidium lysinophilum, Cystobasidium ongulense, Cystobasidium cf. psychroaquaticum, Filobasidium stepposum, Filobasidium wieringae, Fonsecazyma cf. betulae, Holtermanniella cf. wattica, Naganishia adeliensis, Naganishia onofrii, Rhodotorula cf. kratochvilovae, Solicoccozyma gelidoterrea, Solicoccozyma terricola, Tausonia cf. pullulans, Vishniacozyma cf. dimennae, Vishniacozyma tephrensis |
Temporal Coverage
| Start Date / End Date | 2022-05-20 / 2022-09-20 |
|---|
Project Data
This study is part a broader project (FONDECYT 1181510) aiming to study the microbiome of Peltigera lichens and their role in the symbiosis of these lichens. In particular, this study focuses on basidiomycete yeasts associated with thalli and substrates (i.e. soil) of four Peltigera lichen species (P. frigida, P. fuscopratextata, Peltigera ponojensis/monticola 11 and P. rufescens) from the Aysen and Magallanes regions of southern Chile. Two of these species have been reported as endemic to the extreme south of South America (P. frigida and Peltigera ponojensis/monticola 11), which brings special relevance to the study of biodiversity components of poorly studied environments such as those of southern Chile. It is important to note that so far there is no published work that has reported the presence and diversity of basidiomycete yeasts in the thalli of these Peltigera lichen species, and even less in the substrates where they grow. This is the first study on basidiomycete yeasts present in the mycobiome of Peltigera lichens and the substrates where they grow. The results obtained in this project provide information that could contribute to decision-making regarding the management and design of protected areas for the conservation of biodiversity in Chile, including components that are often not considered in this area. In addition, they will provide more evidence on the importance of lichens as complex miniature ecosystems, which harbor a diversity of microbial communities, and as such, the importance of knowing and preserving them. Some of the basidiomycete yeasts reported in association with lichens have a lichen-dependent mode of life, so it is very likely that the lichen species studied are reservoirs of hitherto unreported yeasts.
| Title | Basidiomycetes fungi associated with thalli and substrates of Peltigera lichens from southern Chile |
|---|---|
| Identifier | YEAST_PELT |
| Funding | ANID – FONDECYT 1181510: Selectivity for new symbiotic players in the lichenization process: novel insights into a relationship discovered 150 years ago. ; ANID – Millennium Science Initiative Program – ICN2021_002: Millennium Institute Biodiversity of Antarctic and Subantarctic Ecosystems. ; Beca de Doctorado Nacional ANID 2019 21190058: Basidiomycetes fungi associated with thalli and substrates of Peltigera lichens from southern Chile |
| Study Area Description | Samples of each lichen species (P. frigida, P. fuscopratextata, Peltigera ponojensis/monticola 11, P. rufescens) and their substrates were collected at four sites in southern Chile, two in the Aysen Region: (1) Coyhaique National Reserve and (2) Patagonia National Park, Tamango sector; and two in the Magallanes Region: (3) Karukinka Natural Park and (4) Navarino Island, near Puerto Williams. At each site, two environmental contexts were included, grassland and forest, and each species was collected considering its distribution: P. frigida and P. fuscopratextata in forests, and Peltigera ponojensis/monticola 11 and P. rufescens in grasslands. Samples were collected in different sampling years between 2013 and 2020. These samples are currently part of the Peltigera lichen collection of southern Chile, from the Microbial Ecology Laboratory at Universidad de Chile. The predominant vegetation cover in the sites corresponds to mature and young forests of Nothofagus pumilio and Patagonian steppe. Permits for sampling in the protected areas were obtained from CONAF (Corporación Nacional Forestal). |
| Design Description | The main objective of this project is to determine the diversity of basidiomycete yeasts associated with Peltigera lichens from southern Chile and the specificity of the main mycobiont for them. The first step was the molecular identification of the symbiotic pairs of the lichen species P. frigida, P. fuscopratextata, Peltigera ponojensis/monticola 11 and P. rufescens. Once the study species were identified, we proceeded to evaluate the diversity of basidiomycete yeasts associated with the thalli and substrates of the four Peltigera lichen species. Two methodologies were proposed for this purpose. Massive sequencing of amplicons obtained from thalli and substrates of the four study species with specific primers for basidiomycetes (unpublished) and a culture-dependent method. Yeasts were isolated from the thalli of each lichen species from each study site. Isolation was performed on three different culture media and with an incubation temperature of 10°C in the dark. The isolates were identified by sequencing the ITS marker and subsequent phylogenetic analyses. Therefore, the results obtained from the isolation are complemented with massive sequencing to better describe the diversity of these yeasts in the thalli of the Peltigera lichens studied. |
Sampling Methods
Yeasts were isolated from thalli of four lichen species of the genus Peltigera (P. frigida, P. fuscopraetextata, Peltigera ponojensis/monticola 11, P. rufescens). The thalli were washed twice with sterile distilled water and subsequently macerated. They were seeded on three culture media (Yeast Malt Agar, Potato Dextrose Agar and a minimum agar medium without amino acids) supplemented with antibiotics. Cultures were incubated at 10°C in the dark for up to 60 days. Long-term preservation of yeast isolates was achieved at -20°C using cryotubes with sterile 15% glycerol and a dehydrated gelatin drop method at room temperature with sterile 10% gelatin (Marangon et al., 2003; Baeza et al., 2009). Taxonomic identification was performed by sequencing the ITS marker and subsequent phylogenetic analyses
| Study Extent | The samples of Peltigera species was collected in Region of Aysen del General Carlos Ibañez del Campo and Region of Magallanes y de la Antartica Chilena, Chile. The idendification of the yeast isoleted from the lichens was made betewen may and september of 2022 |
|---|
Method step description:
- The thalli were washed twice with sterile distilled water and subsequently macerated.
- They were seeded on three culture media (Yeast Malt Agar, Potato Dextrose Agar and a minimum agar medium without amino acids) supplemented with antibiotics.
- Cultures were incubated at 10°C in the dark for up to 60 days.
- Long-term preservation of yeast isolates was achieved at -20°C using cryotubes with sterile 15% glycerol and a dehydrated gelatin drop method at room temperature with sterile 10% gelatin.
- Taxonomic identification was performed by sequencing the ITS marker and subsequent phylogenetic analyses.
Collection Data
| Collection Name | Collection of basidiomycetes yeasts isolated from Peltigera lichens |
|---|---|
| Collection Identifier | YEAST_PELT |
| Parent Collection Identifier | https://doi.org/10.15468/ef76hj |
| Specimen preservation methods | Deep frozen, Dried |
|---|
Bibliographic Citations
- Baeza, M., Retamales, P., Sepúlveda, D., Lodato, P., Jiménez, A., & Cifuentes, V. (2009). Isolation, characterization and long term preservation of mutant strains of Xanthophyllomyces dendrorhous. J. Basic Microbiol., 49: 135-141.
- Marangon, A.V., Bertoni, T.A and Kioshima, E.S., (2003). Dehydrated gelatin drops: A good method for fungi maintenance and preservation. Microbiologica, 26, 305– 309. PMID: 12901428.
- Orlando J, Almendras K, Veas-Mattheos K, Pezoa M, Carú M (2023). Peltigera cyanolichens from Southern Chile. Version 1.5. Laboratory of Microbial Ecology (Laboratorio de Ecología Microbiana) - Universidad de Chile. Occurrence dataset https://doi.org/10.15468/ef76hj https://doi.org/10.15468/ef76hj
- Pérez, Y., Serey, N., Lizana, N., Millanes, A. M., Almendras, K., & Orlando, J. (2023). Primer reporte de levaduras basidiomicetes aisladas desde líquenes Peltigera del sur de Chile. XLIV Reunión anual de la Sociedad de Microbiología de Chile, La Serena, Coquimbo, Chile. Zenodo. https://doi.org/10.5281/zenodo.10424087 https://doi.org/10.5281/zenodo.10424087
- Pérez, Y., Serey, N., Millanes, A. M., Almendras, K., & Orlando, J. (2023). Diversidad de hongos aislados de talos de cianolíquenes Peltigera del sur de Chile. 3er Congreso Latinoamericano de Ecología Microbiana (ISME-Lat), Universidad Nacional de Quilmes, Bernal Buenos Aires, Argentina. Zenodo. https://doi.org/10.5281/zenodo.10420231 https://doi.org/10.5281/zenodo.10420231
- Pérez, Y.; Veas-Mattheos, K.; Almendras, K.; Orlando, J. Basidiomycete yeasts in lichens: a new component of lichen symbiosis?. ISME Latino Americano (ISME-LAT), Chile, 2021.
Additional Metadata
| Alternative Identifiers | 6322f1dd-67c5-4938-bc43-ca66ea3cf102 |
|---|---|
| https://gbif-chile.mma.gob.cl/ipt/resource?r=yeast_pelt_basi |